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The use of biofuels is one way of reducing the increasingly visible harmful impact of diesel engines on the environment. At the same time, it is also a way of gradually reducing dependence on dwindling oil reserves. New sources of biodiesel production are currently being sought. At present, new types of plant-based fuels are increasingly being introduced onto the market. Due to their different chemical composition compared to diesel fuel, these fuels may have significantly lower oxidation resistance. Oxidation stability is one of the basic performance properties that is determined for fuels used to power compression ignition engines. The article presents the results of oxidation stability tests using the Anton Paar RapidOxy apparatus, which uses a small-scale accelerated oxidation method in accordance with EN 16091. The tests were carried out on diesel fuel and biofuels of various origins.